Part:BBa_K2401000
Synthethases + Activators for Pyochelin and Yersiniabactin siderophor
This part encodes for the siderophor synthetase HMWP2, this synthetase is responsible for the first part of the non ribosomal synthesis (NRS) of yersiniabactin. The structure of the product is very similar to pyochelin, the secondary siderophor originating from pseudomonas aeruginosa. The only structural difference is a single methyl group, attached to one of the cystein residues. This derivate of pyochelin is called n-Nor-Pyochelin, a derivate with a 30% increased receptor affinity.
The other genes are: 1. 2,3-dihydroxybenzoate-AMP ligase, this protein activates the core substrate of the pyochelin and yersiniabactin synthesis, salicylate. 2. Thiazolinyl imide reductase, this protein is a common catalysator in NRS for peptides including cyclized cysteine, which this reductase forms out of linear cysteine creatin a thiazolin ring. 3. surfactin synthase-activating enzyme, transfers Coenzyme A to a serine residue conserved in all peptidyl carrier proteins which are an essential part of NRS.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 8003
Illegal SapI site found at 8029
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