Composite

Part:BBa_K2401000

Designed by: Shanti Ricke   Group: iGEM17_Hamburg   (2017-10-19)


Synthethases + Activators for Pyochelin and Yersiniabactin siderophor

This part encodes for the siderophor synthetase HMWP2, this synthetase is responsible for the first part of the non ribosomal synthesis (NRS) of yersiniabactin. The structure of the product is very similar to pyochelin, the secondary siderophor originating from pseudomonas aeruginosa. The only structural difference is a single methyl group, attached to one of the cystein residues. This derivate of pyochelin is called n-Nor-Pyochelin, a derivate with a 30% increased receptor affinity.

The other genes are: 1. 2,3-dihydroxybenzoate-AMP ligase, this protein activates the core substrate of the pyochelin and yersiniabactin synthesis, salicylate. 2. Thiazolinyl imide reductase, this protein is a common catalysator in NRS for peptides including cyclized cysteine, which this reductase forms out of linear cysteine creatin a thiazolin ring. 3. surfactin synthase-activating enzyme, transfers Coenzyme A to a serine residue conserved in all peptidyl carrier proteins which are an essential part of NRS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 8003
    Illegal SapI site found at 8029


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Categories
Parameters
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