Composite

Part:BBa_K2387009

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-15)


eYFPn [1-154] and eYFPc[155-238] + araC/pBAD promoter

The N-terminus eYFPn[1-154] and C-terminus of fluorescent protein eYFP eYFPc[155-238]are put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.

It is used as a negative control in analyzing the functioning of inducible promoter araC/pBAD. Furthermore it is used to display the absence of affinity of lone eYFP termini, as a negative for BiFC


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None