Plasmid_Backbone

Part:BBa_K238026

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-10-04)


pMA plasmid

Part BBa_K238024, the lock, was sent to us by Geneart in this plasmid. It differs from pMA-RQ in that it has an extra suffix and 4 extra basepares after the second suffix, being TACT, making it 25bp longer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 23
    Illegal PstI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2417
    Illegal SpeI site found at 2
    Illegal SpeI site found at 23
    Illegal PstI site found at 16
    Illegal PstI site found at 37
    Illegal NotI site found at 9
    Illegal NotI site found at 30
    Illegal NotI site found at 2423
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2417
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2417
    Illegal suffix found at 2
    Illegal SpeI site found at 23
    Illegal PstI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2417
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2432
    Illegal SpeI site found at 2
    Illegal SpeI site found at 23
    Illegal PstI site found at 16
    Illegal PstI site found at 37
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None