Plasmid_Backbone

Part:BBa_K238025

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-10-04)


pMA-RQ plasmid

This is a geneart plasmid in which our antisense key part (BBa_K238005) is constructed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2392
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2398
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2392
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2392
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2392
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2407
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None