Composite

Part:BBa_K238023

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-09-24)


full vanillin production

this is the complete vanillin production. all the necessary genes are put together. this part differs from part K238004 because the lock system is not integrated in it. this part is made for checking the complete vanillin production from tyrosin but can not be regulated through the key/antikey system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 851
    Illegal BamHI site found at 1253
    Illegal BamHI site found at 6255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 594
    Illegal NgoMIV site found at 1638
    Illegal NgoMIV site found at 1646
    Illegal NgoMIV site found at 2396
    Illegal NgoMIV site found at 2569
    Illegal NgoMIV site found at 2971
    Illegal NgoMIV site found at 2975
    Illegal AgeI site found at 541
    Illegal AgeI site found at 710
    Illegal AgeI site found at 4843
    Illegal AgeI site found at 5380
    Illegal AgeI site found at 5841
    Illegal AgeI site found at 6283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 145
    Illegal BsaI site found at 411
    Illegal BsaI.rc site found at 2170
    Illegal BsaI.rc site found at 3760


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Parameters
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