Composite
EF

Part:BBa_K238014

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-09-21)


ligation of ech and fcs

this is the ligation of ech and fcs. these are the last two enzymes in the vanillin production. this is the 'clean' form since there is no promotor or terminator attached. for a complete construct see BBa_K238006

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1963
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 1123
    Illegal AgeI site found at 551
    Illegal AgeI site found at 1088
    Illegal AgeI site found at 1549
    Illegal AgeI site found at 1991
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None