Composite

Part:BBa_K238003

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-15)

Vanillin receptor

VirA/G system is a phenol sensing system originally found in agrobacterium tumefaciens. it binds phenols such as vanillin and the activates gene transcription.

VirA and RpoA both have illegal restriction sites in their original sequence. VirA has two PstI sites and RpoA two EcoRI sites. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA. EcoRI was mutated from GAATTC to GAATTT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 728
    Illegal BglII site found at 1621
    Illegal BglII site found at 4394
    Illegal BamHI site found at 3287
    Illegal XhoI site found at 2841
    Illegal XhoI site found at 2934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1467
    Illegal NgoMIV site found at 4367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1239
    Illegal BsaI site found at 2031
    Illegal BsaI.rc site found at 4023
    Illegal SapI site found at 1713
    Illegal SapI.rc site found at 942


[edit]
Categories
Parameters
n/aVanillin receptor