Part:BBa_K2372008

pRha-RBS-SHV1 for controlled expression of beta-Lactamase

This is a composite of our expression part (BBa_K2372007) and SHV-1 (BBa_K2372004) a broad-spectrum beta-lactamase. The expression is tightly controlled and dependent on addition of L-Rhamnose.

Our composite part was designed by combining pRha (Part: BBa_K914003), a Ribosome Binding Site (RBS) (Part: BBa_B0034) and SHV-1 (Part: BBa_K2372004). The information for pRha and the RBS are available at the iGEM website. The promoter pRha is used to control the expression of the target gene. The RBS is part of the mRNA and binds upstream (5’ End) to the DNA. A RBSs task is to position the mRNA at the right place. The efficiency of the bonding determines how well the translation is accomplished. SHV-1 is an enzyme of the β-lactamase family typically found in the Klebsiella.Most of the antibiotic resistances especially of K. pneumonia have been attributed to SHV-1. SHV-1 belongs to a group of so called Extended-spectrum β-lactamases (ESBLs). They are known for inhibiting antibiotics by hydrolysis. Most problematic is that ESBLs are encoded on plasmids and therefore, can be exchanged between bacteria.

References
Shaikh, S., Fatima, J., Shakil, S., Rizvi, S. M., & Kamal, M. A. (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi J Biol Sci, 22(1), 90-101. doi:10.1016/j.sjbs.2014.08.002

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 294
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 811
  
• 1000
  COMPATIBLE WITH RFC[1000]