Part:BBa_K237002

Constitutive RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species [1]. RIP effectively works as an antibiotic in its inhibition of bacterial biofilm-formation. It is important to note that even though it has the effect of an antibiotic [1] on bacterial load, the mechanism is a novel one, and doesn't appear to induce bacterial resistance [1].

RIP has the sequence YSPXTNF(where X can be a cysteine, a tryptophan, or a modified amino acid) and has been tested extensively in a synthetic (as in chemical synthesis) form for ability to inhibit bacterial infections in mice [1] and biofilm formation on human cells [2].

This generators function is to constitutively express RIP cytoplasmatically for later harvest.

We have shown by northern blotting that RIP is indeed transcribed via this plasmid.

Usage and Biology

This generator is under constitutive expression by a TetR repressible promoter BBa_R0040.

Our examinations of this brick indicate that while RIP is expressed, it is not transported into the medium. This is to be expected. Due to this lack of exportation, this brick will serve best as a production vector in cells that will have to lysed to obtain the peptide.

References

[1] Suppression of Drug-Resistant Staphylococcal Infections by the Quorum-Sensing Inhibitor RNAIII-Inhibiting Peptide. Journal of Infectious Diseases [serial on the Internet]. (2004, July 15), [cited October 21, 2009]; 190(2): 318-320. Available from: Academic Search Premier. [http://www.ncbi.nlm.nih.gov/pubmed/15216467]

[2] Prevention of Staphylococcus aureus biofilm on dialysis catheters and adherence to human cells. Balaban N, Gov Y, Bitler A, Boelaert JR. Kidney Int 2003; 63:340–5. [http://ncbi.nlm.nih.gov/pubmed/12472801]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]