Composite

Part:BBa_K2365049

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-14)


PDC1 promoter-CYC1 terminator

Between the PDC1 promoter and cyc1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289


Pdc1 pdc1-cyc1.jpeg

We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm

U-disk test.jpg
酵母荧光.jpg
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