Regulatory

Part:BBa_K2365040

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-14)


PGK1 promoter

PGK1 Yeast promoter Constitutive expression

Phosphoglycerate kinase, phosphoglycerate kinase promoter, transcription start key enzyme of glycolysis, and each species to enzyme must survive, the deficiency of the enzyme can cause organism metabolism disorder, as a strong promoter in gene engineering


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

启动子全部.jpeg

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

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Parameters
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