Part:BBa_K2360007

pChrB-ChrB

It is special operon which can make a specific reponse to chromium ions.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 136
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 911
  
• 1000
  COMPATIBLE WITH RFC[1000]

Figure 1. Bio-circuit of BBa_K2360007

Lysis gene expression

We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions. Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.

Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions.
we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr⁶⁺, which showed this devices did not work as we expected.