Coding

Part:BBa_K2354011

Designed by: CHAN , WEI-JEN   Group: iGEM17_NTHU_Taiwan   (2017-10-21)


INP-ER-alpha HS

This part is codon optimized for expression in E.coli (Escherichia coli). Estrogen receptor can bind to any EDCs and this binding ability is a very important property for the purpose of detection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 631
    Illegal AgeI site found at 1049
    Illegal AgeI site found at 2080
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None