Project

Part:BBa_K235035

Designed by: Natasha Tuskovich   Group: iGEM09_VictoriaBC   (2009-10-20)

[K235033][K235032] (constituitive promoter+32°C RBS thermometer+LacI+stop+pLac+RBS+GFP+stop)

This part was designed to produce GFP below 32°C but was not debugged in time for the 2009 competition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2303


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Categories
Parameters
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