Project

Part:BBa_K235027

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[K235024][K235000] (NAND gate control test, arabinose positive control)

This BioBrick is a control for the NAND gate (K235025). This system constitutively produces the ribolocked mRNA for the λcI repressor protein. It can therefore be used as a positive control for the arabinose segment of the system; only the presence or absence of IPTG should change the level of mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 941
    Illegal NheI site found at 964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2152


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Categories
Parameters
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