Project

Part:BBa_K235026

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[K235022][K235001] (NAND gate control test, pLac positive control)

This BioBrick is a control for the NAND gate (K235025). This system constitutively produces the ribokey. It can therefore be used as a positive control for the lactose segment of the system; only the presence or absence of arabinose should change the level of mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2139
    Illegal NheI site found at 3128
    Illegal NheI site found at 3151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2078
    Illegal BamHI site found at 3285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3162
    Illegal SapI site found at 1895


[edit]
Categories
Parameters
None