Project

Part:BBa_K235025

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)

[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

This BioBrick replicates the function of a NAND logic gate (http://en.wikipedia.org/wiki/NAND_gate). The inputs are a) an inducer for the lactose operon, and b) an inducer for the arabinose operon. The output is the production of the mCherry fluorescent protein (J06504).

IPTG in medium? Arabinose as best carbon source? mCherry produced?
no no yes
no yes yes
yes no yes
yes yes no

This system is sensitive to the cellular production of λcI repressor protein. Use with caution in cell strains with arabinose- or lactose-related sensitivity or insensitivity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2078
    Illegal BamHI site found at 3450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3327
    Illegal SapI site found at 1895


[edit]
Categories
Parameters
None