Reporter

Part:BBa_K235022

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[K235018][K235019] (mCherry generator, pAra-controlled ribokey-mediated signal inversion)

This BioBrick generates the mCherry fluorescent protein (J06504), under the control of the λcI-repressed promoter. When the cell is metabolising an inducer for the arabinose promoter (I0500), the mRNA for the λcI repressor protein (C0051) is produced. If a ribokey (i.e., J23066) is present in addition to an inducer, then production of mCherry will halt.

This system is sensitive to the cellular production of λcI repressor protein. Use with caution in cell strains with arabinose-related sensitivity or insensitivity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2078
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1895


[edit]
Categories
Parameters
None