Generator

Part:BBa_K235019

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[K235016][K235003] (ribokey-mediated pAra-controlled lambda repressor generator)

This BioBrick generates mRNA for the λcI repressor protein when the arabinose promoter is induced by arabinose or an analogue. The protein is only translated when a ribokey (i.e., J23066) is present in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None