Composite

Part:BBa_K2350024

Designed by: YU-HSIANG LIN   Group: iGEM17_NYMU-Taipei   (2017-10-21)


pPIGBACK+PrbcL+MelA+rrnb

To transform and express foreign gene in cyanobacteria, we ligate our plasmid with unique vector designed by ourselves. The promoter and terminator chosen is compatible to cyanobacteria, and the well-designed backbone aims to trigger homologous recombination in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1861
    Illegal BamHI site found at 2158
    Illegal BamHI site found at 5166
    Illegal BamHI site found at 6096
    Illegal XhoI site found at 1096
    Illegal XhoI site found at 4288
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2079
    Illegal AgeI site found at 5360
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 950
    Illegal BsaI site found at 1761
    Illegal BsaI site found at 6298
    Illegal BsaI.rc site found at 4444
    Illegal BsaI.rc site found at 4735


BBa_K2350024-plate_image_1.png

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