Part:BBa_K2350014
Lycopene cyclase upstream + Ampicillin Resistance + Lycopene cyclase downstream
In order to present Lycopene in Cyanobacterium Synechococcus sp Strain PCC7942, we use the strategy “Gene Knock Out”. The function of this part is to execute "Gene Knock Out". When the constructed plasmid containing” Lycopene-US + AmpR + Lycopene-DS” is transferred into PCC7942, there is a probability to occur Homologous Recombination in the genome of PCC7942 through this transferred plasmid. The Lycopene cyclase gene (CrtL) will be replaced by Ampicillin Resistance gene(AmpR) in Homologous Recombination. And then, the “Gene Knock Out” has been completed.
The following statement is a brief introduction to the reason why we use "Gene Knock Out": Lycopene is an intermediate in the biosynthesis pathway of beta-carotenoids in Cyanobacterium Synechococcus sp Strain PCC7942. There is an enzyme, called Lycopene cyclase, converts acyclic hydrocarbon Lycopene into the bicyclic Beta-carotene. Once we can knock out the Lycopene cyclase gene (CrtL), the Lycopene cyclase won’t exist. If the lycopene cyclase doesn’t exist, the Lycopene will remain and not be transferred into other compounds.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3326
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2656
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2069
Illegal BsaI site found at 2649
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