Part:BBa_K2350008
Lycopene cyclase downstream in PCC7942
In order to present Lycopene in Cyanobacterium Synechococcus sp Strain PCC7942, we use the strategy：“Gene Knock Out”. This part provides the sequence of the downstream of Lycopene cyclase in Cyanobacterium Synechococcus sp Strain PCC7942 (we call it ” Lycopene-DS”) Lycopene-DS is one of the parts to construct the plasmid executing "Gene Knock Out".
When the plasmid has been constructed completely, it contains ” Lycopene-US + AmpR + Lycopene-DS”.(As you can see, Part BBa_K2350014) There is a probability to occur Homologous Recombination after transferring this plasmid into PCC7942. The Lycopene cyclase gene (CrtL) will be replaced by Ampicillin Resistance gene(AmpR) in Homologous Recombination. And then, the “Gene Knock Out” is completed.
The following statement is a brief introduction to the reason why we use "Gene Knock Out" : Lycopene is an intermediate in the biosynthesis pathway of beta-carotenoids in Cyanobacterium Synechococcus sp Strain PCC7942. There is an enzyme, called Lycopene cyclase, converts acyclic hydrocarbon Lycopene into the bicyclic Beta-carotene. Once we can knock out the Lycopene cyclase gene (CrtL), the Lycopene cyclase won’t exist. If the lycopene cyclase doesn’t exist, the Lycopene will remain and not be transferred into other compounds.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1112
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 442
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 435
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