Coding

Part:BBa_K2347003

Designed by: Zexun Wu   Group: iGEM17_NPU-China   (2017-10-19)


NADH->NAD+

Can oxidize either NADH or NADPH with a preference for NADH. Can catalyze electron transfer from NADH to various electron acceptors which include, in addition to molecular oxygen, cytochrome c, 2,6 dichlorphenolindophenol, methylene blue, ferricyanide or P-nitroblue tetrazolium.
Catalytic activity
NADH + acceptor = NAD++ reduced acceptor.
Cofactor
FMNNote: Binds 1 FMN per subunit.
pH dependence
Optimum pH is 5.0.
Temperature dependencei
Thermostable.

TO LEARN MORE: http://www.uniprot.org/uniprot/Q60049

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 52
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 173


[edit]
Categories
Parameters
None