Coding

Part:BBa_K2347001

Designed by: Zexun Wu   Group: iGEM17_NPU-China   (2017-10-15)


Glycerol->DHA(Dihydroxyacetone)

Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Is also able to use various diols as substrates, such as propan-1,2-diol, butan-2,3-diol, ethan-1,2-diol, and 3-mercapto-1,2-dihydroxypropane.1 Publication
Catalytic activity
Glycerol + NAD+= glycerone + NADH.
Cofactor
Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.
Kinetics
KM=3.8 mM for glycerol1
KM=121 uM for NAD+
pH dependence
Optimum pH is 6.0-8.5.

TO LEARN MORE: http://www.uniprot.org/uniprot/P32816

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None