Device
NisAp

Part:BBa_K2346003

Designed by: Li Jianan   Group: iGEM17_HFLS_H2Z_Hangzhou   (2017-10-23)


Nitrate/nitrite responsive promoter with amplifier

This is a composite part and a functional improved device for Part:BBa_K216005(https://parts.igem.org/Part:BBa_K216005). The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users. We ligated this part with a GFP+terminator. BBa K2346003.png Conclusion As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None