Tag

Part:BBa_K2342001

Designed by: Ushanandini Mohanraj   Group: iGEM17_Aalto-Helsinki   (2017-10-25)


SUMO fusion tag, Smt3

Employing of Smt3 tag is beneficial in several aspects in expression of recombinant proteins. Ulp1 is a robust enzyme that specifically facilitates cleavage of Smt3, leaving protein of interest unbound to tag. SUMO tag is known for its effect on solubility and preventing inclusion bodies of fusion peptide, significantly easing purification step(1).


References-

1) Malakhov, M. P., Mattern, M. R., Malakhova, O. A., Drinker, M., Weeks, S. D., & Butt, T. R. (2004). SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. Journal of structural and functional genomics, 5(1), 75-86. 5)Burian, M., & Schittek, B. (2015). The secrets of dermcidin action. International Journal of Medical Microbiology, 305(2), 283-286.


Usage and Biology

In our project we demonstrate an effective SUMO fusion based production system for antimicrobial peptides (AMPs).

For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008, BBa_K2342009

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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