Plasmid

Part:BBa_K233324

Designed by: swetha srinivasan,samit watve, mandar phatak, chinar patil   Group: iGEM09_IBB_Pune   (2009-10-19)

pIDTSmart-KAN Vector

This is a plasmid with a

1) pUC 18 derived origin of replication

2) Gene for kanamycin Resistance

3) M13 forward and reverse primer sites

4) Contains biobrick prefix and suffix

Snapshot of pIDT Smart-Kan vector containing YcdB gene





















Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1925
    Illegal suffix found in sequence at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1925
    Illegal SpeI site found at 3
    Illegal PstI site found at 17
    Illegal NotI site found at 10
    Illegal NotI site found at 1931
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1925
    Illegal suffix found in sequence at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1925
    Illegal XbaI site found at 1940
    Illegal SpeI site found at 3
    Illegal PstI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None