Part:BBa_K233321

Turing Machine - Unary Adder 2

This part is designed to respond to two chemical inputs 1) Lactose/ iptg 2) AHL (acyl homoserine lactone and its derivatives used in quorum sensing)

If cells containing this construct are placed in an environment containing lactose/ IPTG ONLY, the cells show no difference.

If the cells are placed in an environment containing AHL ONLY, they begin to produce their own AHL. This is acheived in the following manner. AHL binds to LuxR (which is constitutively produced). The AHL-LuxR complex, activates the promoter pLuxR. This initiates the transcription of the LuxI gene responsible for production of AHL. The reporter gene (Lysis Cassette) however is not transcribed since the repressor protein is bound to LacO binding site (downstream of the pLuxR promoter) in absence of Lactose. This prevents transcription of the reporter gene since RNA polymerase is unable to bind to the promoter.

In presence of BOTH AHL and Lactose. The reporter gene is ALSO activated along with the gene for AHL production. This causes cell lysis.

The construct if coded in a proper manner responds to chemical inputs AHL and Lactose, and behaves like a Turing Machine (Unary Adder).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1982
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1982
  Illegal NheI site found at 877
  Illegal NheI site found at 900
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1982
  Illegal BglII site found at 719
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1982
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1982
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2731
  Illegal BsaI.rc site found at 1855