DNA
comEF

Part:BBa_K233303

Designed by: Praveen Kishore Sahu, Amrita Oak   Group: iGEM09_IBB_Pune   (2009-09-20)


Competence factors E & F

com E and F genes are found in Acinetobacter BD413 sp. The ORF of the two genes has a size of ~1kb. The pillin like competence factors codes byn these two genes are involved in natural DNA translocation and uptake from surrounding and works in conjunction with comP, conferring natural competence to the bacterium. Com F gene mutants are found to be 1000 times less competent. (Busch et.al 1999.)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 965
    Illegal XbaI site found at 195
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 966
    Illegal PstI site found at 980
    Illegal NotI site found at 7
    Illegal NotI site found at 973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 966
    Illegal XbaI site found at 195
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal XbaI site found at 195
    Illegal SpeI site found at 966
    Illegal PstI site found at 980
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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