Coding

Part:BBa_K2331000

Designed by: Jacob Sicheri   Group: iGEM17_Dalhousie   (2017-10-17)


Alpha-L-Arabinofuranosidase

Alpha-L-Arabinofuranosidases cleave terminal non-reducing alpha-L-arabinofuranoside residues, which can be found in hemicellulose, alkyl glycosides and other molecules. Our main vision for the part was for breaking down hemicellulose found in softwood and hardwood. This part should be able to free up hemicellulose polymers for easier degradation by enzymes such as endo/exo-xylanases, and beta-xylanases (in the case of xylose hemicellulose polymers).

This part is codon optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1413
    Illegal SapI.rc site found at 1336


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Categories
Parameters
None