Regulatory

Part:BBa_K233004

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-07-10)

lac operator

This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI.

A completely symmetric DNA segment has been constructed that binds the lactose repressor of Escherichia coli 10- fold more tightly than does the natural lactose operator sequence. This tight-binding operator is an inverted repeat of a 15-base-pair segment from the left half of the natural operator sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None