Composite
Co-op

Part:BBa_K233002

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-07-10)

Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

This is a module that contains the following components: 1. A constituive promoter 2. RBS (ribosome binding site) 3. An export tag viz. YcdB 4. Lac Z gene (Gene for enzyme B-galactosidase) 5. A reporter gene viz. GFP 6. TERM


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3918


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Categories
Parameters
None