Composite

Part:BBa_K233001

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-07-10)

Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

This is a device that contains the following components:

1. An inducible lac promoter with the operator( lacI binding sites)

2. RBS (ribosome binding site)

3. An export tag viz. torA

4. Lac Z gene (Gene for enzyme B-galactosidase)

5. A reporter gene viz. GFP

6. TERM

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4099


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