Device

Part:BBa_K2326006

Designed by: Zhongxiu Hu   Group: iGEM17_BNDS_China   (2017-10-24)


LacI - pTac - gadA - GFP - Terminator

This ribosomal binding site that has a relative translation rate of 300 is calculated via RBS calculator from Salis Lab at Penn State University for the transcriptional Regulator of GAD operon in Lactobacillus brevis NCL912.


Introduction

800px-Fig_3_Third_Approach_BBa_K2326005_%26_BBa_K2326006.png

Fig. 1 Thrid Approach: BBa_K2326005 & BBa_K2326005

BBa_K2326006 enabled us to quantify the expression rate of gadA since the protein is synthesized with GFP.

Fig_10_Relative_Concentration.png

The concentration of GFP of recombinant E. coli Nissle (RPG- BBa_K2326006) was higher than the negative control, indicating that the fusion protein of GAD and GFP was expressed. Thus, GAD could be expressed in E. coli Nissle (RPG-BBa_K2326005).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1443
    Illegal AgeI site found at 1522
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3469


[edit]
Categories
//awards/composite_part
//cds/biosynthesis
Parameters
None