Coding

Part:BBa_K2324016

Designed by: Daniel Barber   Group: iGEM17_Exeter   (2017-11-01)


fim AIC

This part includes the coding sequences for proteins FimA, FimI and FimC that form part of the E. coli fim operon (Le Trong et al 2010). The complete operon fimAICDFGH allows for biosynthesis of pili, small hair-like protein structures on the surface of bacterial cells. This part is included in the composite parts http://parts.igem.org/Part:BBa_K2324012 and http://parts.igem.org/Part:BBa_K2324015.


References

Le Trong, I., Aprikian, P., Kidd, B. A., Thomas, W. E., Sokurenko, E. V., and Stenkamp, R. E. (2010) Donor strand exchange and conformational changes during E. coli fimbrial formation. Journal of Structural Biology 172, 380–388.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 868
    Illegal AgeI site found at 899
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None