Signalling

Part:BBa_K2323007

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-26)


GFP-LVA

This GFP (E0040) is tagged with a protein degradation tag (pdt) called LVA. This causes the protein to degrade in E.coli with a first-order rate of 0.0126 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

This biobrick is part of a collection of degradation tags, characterized on the page BBa_K2323003. See also BBa_K2323005, BBa_K2323006, BBa_K2323008.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724

Plasmid composition

The collection is cloned from BBa_I13522 with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. (read-only benchling map) Cloning was confirmed with sequencing.


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Categories
Parameters
None