Composite

Part:BBa_K2323001

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)


Leptotrichia wadei Cas13a with a His-Tag and a SUMO-tag for protein purification.

Uses a 6xHis/Twin Strep (BBa_K2323011) to allow purification and a SUMO-tag to improve solubility (BBa_K2323012) and as a cleavable tag of Lwa Cas13a ((BBa_K2323000)) for in-vitro use.

For characterization, see the full construct: BBa_K2323004.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1736
    Illegal BglII site found at 3416
    Illegal BamHI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2815

Plasmid composition

The Biobrick BBa_K2323000 is preceded by a 6xHis/Twin Strep and a SUMO tag.(read-only benchling map)

The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)


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