Composite

Part:BBa_K2323000

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)


Lwa Cas13a + Tphi terminator

Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.

The coding sequence is followed by Tphi terminator.

For characterization, see the full construct: BBa_K2323004. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 2966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2365

Plasmid composition

The Biobrick contains the Lwa coding sequence and the Tphi terminator.(read-only benchling map)

The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)


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