Lwa Cas13a + Tphi terminator
Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.
The coding sequence is followed by Tphi terminator.
For characterization, see the full construct: BBa_K2323004. Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1286
Illegal BglII site found at 2966
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 2365
The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)