Reporter

Part:BBa_K2321002

Designed by: Xianghui Zhao   Group: iGEM17_SDU_CHINA   (2017-10-24)


firefly luciferase reporter

Firefly luciferase gene serving as a reporter, was originally on the pGL3-Basic vector. This vector provides a basis for the quantitative analysis of factors that poteneially regulate mammalian gene expression. These factors may be cis-acting elements, such as promoters and enhancers, or trans-acting factors, such as various DNA-binding proteins. In our project, hTERT/survivin promoters were inserted respectively into pGL3-Basic vector to determine the startup efficiency of promoter by detecting the expression of firefly luciferase gene with the fluorescene intensity measurement.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


[edit]
Categories
Parameters
None