Scar
Part:BBa_K2319001
Designed by: Raj Magesh Gauthaman Group: iGEM17_IISc-Bangalore (2017-10-24)
HindIII+ATG+AgeI scar
This part is a short (15 bp) scar comprising a start codon inserted between the HindIII and AgeI restriction sites. We used this scar sequence between our promoter and protein coding sequence (a protein domain that lacks its native ATG). This design allows for simple, rapid assembly of different fusion proteins under the same expression system (for example, we used the T7 expression system).
To use these sites effectively to assembly multiple fusion proteins under the expression system of your choice, this part must be used in conjunction with our other part BBa_K2319003, which includes an NheI restriction site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |