Part:BBa_K2310900
Formaldehyde Dismutase
This is an improved version of BBa_K1960103. Formaldehyde dismutase can catalyze the reaction of the disproportionate of aldehyde group, for example, formaldehyde can be catalyze into methanol and carbon dioxide. The expression of this BioBrick part is much better than BBa_K1960103.
The SDS-PAGE for BBa_K1960103 and BBa_K2310900 in plasmid pET-28a
(Both of them with a His-tag is about 50kDa)
Meanwhile, this part can also replace BBa_K1334015, BBa_K1334016 and BBa_K1334017 which have the similar functions.
Usage and Biology
Formaldehyde dismutase can catalyze the reaction of the disproportionate of aldehyde group, for example, formaldehyde can be catalyze into methanol and carbon dioxide. The coding sequences were cloned into plasmid pET-28a, and induced by IPTG. After a 6 hours inducing with IPTG, the bacteria were resuspended with formaldehyde solution, and incubated at 28℃, assay the content of formaldehyde once an hour.
The enzyme activity assay of BBa_K1960103 and BBa_K2310900
(Both of them with a His-tag is about 50kDa)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 208
Illegal AgeI site found at 520
Illegal AgeI site found at 1003 - 1000COMPATIBLE WITH RFC[1000]
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