DNA

Part:BBa_K2308001

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-04)


knock out gene crtB on genome

This part is used to knock out the gene crtB in Rhodobacter Sphaeroides. The part is composed of 415bp of upstream DNA sequence and 434bp of downstream DNA sequences, and crtB can be knocked out by homologous recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 47
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 162
    Illegal SapI site found at 779


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Categories
Parameters
None