Part:BBa_K2305000

strong promoter, cycle amplification system and GFP/RFP dual fluorescent system

Strong constitutive promoter pcat and promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.

Agarose gel electrophoresis

We calculated the new part’s base number to be 4739bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

Fig.1

DNA sequencing

The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.

Characterization map

Fig.The Growth curve of E.coil.

Fig.The GFP curve of E.coil

Fig.The RFP curve of E.coil

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1591
  Illegal BglII site found at 2770
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4451
  Illegal AgeI site found at 4563
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 64
  Illegal BsaI.rc site found at 3449