Regulatory

Part:BBa_K2304001

Designed by: Tong Bian   Group: iGEM17_HBUT-China   (2017-09-27)


It's a promoter gene

This part is a DNA sequence of a promoter.Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro.So downstream gene after pncrA can be repressed by NcrB.But when there is nickel ion,NcrB will combine with the nickel ion and change its shape,and then the downstream gene after pncrA can be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologyLeptospirillum ferriphilum
strainUBK03