Device

Part:BBa_K228873

Designed by: Haoqian Zhang   Group: iGEM09_PKU_Beijing   (2009-09-22)


AND GATE AraC+RBS(J61100)+T7ptag+Sal+SupD



This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team

PKU AND GATE FOR PARTS.png

This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag.

For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
    Illegal AgeI site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1096


[edit]
Categories
Parameters
n/aAND GATE AraC+RBS(J61100)+T7ptag+Sal+SupD