Composite

Part:BBa_K2284010

Designed by: Eleanor Rose Boardman   Group: iGEM17_UNOTT   (2017-10-26)


P5 targetable RFP reporter

This is the fifth in a series of promoters that team UNOTT made based on a Nielsen and Voigt paper from 2014. It is submitted as part of a unit where the promoter is targetable by gRNAs found in range (BBa_K2284001-005) where gRNA 1 targets the brick containing P1 promoter, gRNA 2 targets the brick containing P2 promoter etc. The brick contains RFP with a strong RBS and terminator B1002. Other bricks are in part range (BBa_K2284006-11).All data on http://2017.igem.org/Team:UNOTT/Experiments wiki page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 608
    Illegal AgeI site found at 720
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None