Generator

Part:BBa_K228104

Designed by: Shan Shen   Group: iGEM09_PKU_Beijing   (2009-09-19)


LacP(R0010) + RBS(J61100) + T7ptag(K228000) + terminator(B0015)

T7 polymerase with an amber mutation coding gene is constructed after Lac promoter which would be triggered by IPTG. T7 polymerase can be well expressed in the exsitence of SupD tRNA. Also, the expression of the T7 polymerase can be regulated by RBS. It is one of the LacP-rbs-T7ptag-terminator family members, ditinguished by different rbs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLacP(R0010) + RBS(J61100) + T7ptag(K228000) + terminator(B0015)