Reporter

Part:BBa_K228098

Designed by: Shan Shen   Group: iGEM09_PKU_Beijing   (2009-09-22)


T7 promoter+medium RBS+GFP

It is a report system to test the AND GATE made up of SupD tRNA coding gene and T7 polymerase with a amber mutation. Test the strength of the fluorescence of the bacteria after the cotransfromation with the AND GATE can directly reflect the features of the AND GATE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 717


[edit]
Categories
Parameters
n/aT7 promoter+medium RBS+GFP