Device

Part:BBa_K228040

Designed by: Gao Rencheng   Group: iGEM09_PKU_Beijing   (2009-09-22)


AND Gate LuxR-rbs(B0032)-T7ptag-AraC-SupD



This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team

PKU AND GATE FOR PARTS.png

This is a AND GATE in the Ecoli stain DH5a. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228038-K228045), which are different in the RBS of T7ptag.

For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5212
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
n/aAND Gate LuxR-rbs(B0032)-T7ptag-AraC-SupD