Coding

Part:BBa_K228000

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-08-25)

T7ptag(T7polymerase with amber mutation)

T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor, BBa_K228001 SupD-tRNA, it can be translated into functional T7 polymerase.

T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF). They kindly provided us with the plasmids. And that's the source where we make this standard part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference Anderson JC, Voigt CA, and Arkin AP. Environmental signal integration by a modular AND gate. Molecular Systems Biology. 2007, 3:133


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Categories
Parameters
n/aT7ptag(T7polymerase with amber mutation)